96th DOG Annual Meeting, 1998

V332

EXPRESSION OF DIFFERENT MATRIX PROTEINS AND PROTO-ONCOGENE C-ETS1 IN NON FIBROUS TYPE PTERYGIA

W. Breipohl1, W. Naib-Majani1, I. Theuerkauf2, H.W. Kaiser3, U. Pleyer4, N. Wernert2

Problems in pterygial growth include the still unknown causative factors in etiology of and the high percentage of recurrences after therapeutical intervention.

Aims: This study was designed to detail our knowledge of biochemical data on the proteinaceous composition of pterygial stroma and its eventual interaction with the blood vessels.

Methods: Methods for matrix protein characterization and localization involved the application of fluorescinated antibodies against a panel of cell membrane and matrix associated proteins (collagens as compared to integrins, cadherins, cytokeratins, fibronectin, laminin) as well as endothelial related Etsl transcription factor.

Results: Results document detailed regional variety in the expression of the mentioned proteins. E.g., collagen type IV – but not I – is preferentially localized around blood vessels while the Ets1 transcription factor is strongly expressed in the endothelial cells. Data for the other proteins differed from these patterns and amongst each other.

Conclucions: Taken together, the data suggest parallels in pterygial pathogenesis with matrix-endothelial interactions found under physiologic (placentation) and pathologic (tumor vascularization) conditions elsewhere.

Departments of:

1 Experimental Ophthalmology,

2 Pathology and

3 Dermatology University of Bonn, Bonn, Germany

4 Ophthalmology, Charité, Berlin, Germany


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