96th DOG Annual Meeting, 1998

R244

EXPRESSION OF HLA-A, B, C AND G RNA IN OCULAR TISSUES

P.Y. Robert1,2, C. Lasalmonie1, M. Cogne1, J.P. Adenis2, C. Hartmann3, M. Drouet 1

Purpose: Evaluation of the presence in adult eye of HLA-A, -B, -C and HLA-G transcripts.

Methods: RNA isolation and specific RT-PCR was performed from frozen ocular tissues, harvested from 4 cadavers. and from first trimester trophoblast and JEG3 cell line. Specificity was assessed by hybridization and sequencing.

Results: HLA-A, -B, -C transcripts ivere found in all tissues. HLA-G transcripts were found in trophoblast and JEG 3, by not in ocular tissues.

Discussion: Classical HLA class I expression is confirmed in the eye. The absence of HLA-G subsides its interest as an histocompatibility antigen or an actor of the immune privilege of the eye.

1ED CNRS J 0118, Medical University

2Department of ophthalmology, CHRU Dupuytren, 2 av Martin Luther King, 87042 Limoges Cedex, France

3Hopital Universitare De La Charite Berlin Allemagne


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