IN VIVO EXPRESSION OF INTERCELLULAR CELL ADHESION MOLECULE-1 (ICAM-1) IN UVEAL MELANOMA
G. Anastassiou, H. Schilling, S. Djakovic, A. Heiligenhaus, K.-P.Steuhl
Cell adhesion molecules are strongly implicated in tumor biological and immunological properties. Although the expression pattern of ICAM-1 on most tumor cells is already investigated very little is known about the expression of ICAM-1 on uveal melanoma cells.
Methods: We performed immunhistochemical staining with anti-ICAM-1 (Clone P2A4, Chemicon Int.; USA) mAb on 90 paraffin embedded tumor tissues. The results of the staining were subsequently compared with the histopathological features, i.e., histological type, tumor greatest diameter, ciliary body involvement, scleral infiltration, extraocular growth, and clinical features, i.e., age, male, metastatic spread, metastasis-free time and overall survival, of the tumors. For statistical analysis we employed the Pearson s chi-square-test, Fisher's exact-test and Kaplan-Meier survival-test.
Results: All tumors stained positive with anti-ICAM-1. Almost 100% positivity was observed in 73 tumors (81%). In 14 tumors (15.5%) a portion of 50-75% of tumor cells stained positive and in additional 3 tumors (3.5%) only 25-50% of tumor cells stained positive. Tumors with more than 25% of tumor cells stained negative with anti-ICAM-1 showed a significantly higher frequency of metastatic spread (p< .006) and extraocular growth (p< .002) as well as a worst overall survival (median survival: 20 months) when compared with tumors stained almost 100% positive with anti-ICAM-1 (median survival: 112 months).
Conclusions: Our results suggest the implication of ICAM-1 in uveal melanoma pathology since alteration of ICAM-1-expression on tumor cells correlated with metastatic spread and consequently short overall survival.
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